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Research Article

Testing Biochemistry Revisited: How In Vivo Metabolism Can Be Understood from In Vitro Enzyme Kinetics

  • Karen van Eunen,

    Affiliations: Department of Molecular Cell Physiology, VU University Amsterdam, Amsterdam, The Netherlands, Kluyver Centre for Genomics of Industrial Fermentation, Delft, The Netherlands, Department of Pediatrics, Center for Liver, Digestive and Metabolic Diseases, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands

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  • José A. L. Kiewiet,

    Affiliations: Department of Molecular Cell Physiology, VU University Amsterdam, Amsterdam, The Netherlands, Kluyver Centre for Genomics of Industrial Fermentation, Delft, The Netherlands

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  • Hans V. Westerhoff,

    Affiliations: Department of Molecular Cell Physiology, VU University Amsterdam, Amsterdam, The Netherlands, Kluyver Centre for Genomics of Industrial Fermentation, Delft, The Netherlands, Manchester Centre for Integrative Systems Biology, Manchester Interdisciplinary BioCentre, The University of Manchester, Manchester, United Kingdom, Synthetic Systems Biology, Netherlands Institute for Systems Biology, University of Amsterdam, Amsterdam, The Netherlands

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  • Barbara M. Bakker mail

    b.m.bakker@med.umcg.nl

    Affiliations: Department of Molecular Cell Physiology, VU University Amsterdam, Amsterdam, The Netherlands, Kluyver Centre for Genomics of Industrial Fermentation, Delft, The Netherlands, Department of Pediatrics, Center for Liver, Digestive and Metabolic Diseases, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands

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  • Published: April 26, 2012
  • DOI: 10.1371/journal.pcbi.1002483

About the Authors

Karen van Eunen, José A. L. Kiewiet, Hans V. Westerhoff, Barbara M. Bakker
Department of Molecular Cell Physiology, VU University Amsterdam, Amsterdam, The Netherlands
Karen van Eunen, José A. L. Kiewiet, Hans V. Westerhoff, Barbara M. Bakker
Kluyver Centre for Genomics of Industrial Fermentation, Delft, The Netherlands
Karen van Eunen, Barbara M. Bakker
Department of Pediatrics, Center for Liver, Digestive and Metabolic Diseases, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands
Hans V. Westerhoff
Manchester Centre for Integrative Systems Biology, Manchester Interdisciplinary BioCentre, The University of Manchester, Manchester, United Kingdom
Hans V. Westerhoff
Synthetic Systems Biology, Netherlands Institute for Systems Biology, University of Amsterdam, Amsterdam, The Netherlands

Corresponding Author

Email: b.m.bakker@med.umcg.nl

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: KVE BMB. Performed the experiments: KVE JALK. Analyzed the data: KVE JALK. Wrote the paper: KVE HVW BMB.