Tuning Promoter Strength through RNA Polymerase Binding Site Design in Escherichia coli

Robert C. Brewster; Daniel L. Jones; Rob Phillips

doi:10.1371/journal.pcbi.1002811

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Open Access

Peer-reviewed

Research Article

Tuning Promoter Strength through RNA Polymerase Binding Site Design in Escherichia coli

Robert C. Brewster ,

Contributed equally to this work with: Robert C. Brewster, Daniel L. Jones

Affiliation Department of Applied Physics, California Institute of Technology, Pasadena, California, United States of America
⨯
Daniel L. Jones ,

Contributed equally to this work with: Robert C. Brewster, Daniel L. Jones

Affiliation Department of Applied Physics, California Institute of Technology, Pasadena, California, United States of America
⨯
Rob Phillips

* E-mail: phillips@pboc.caltech.edu

Affiliations Department of Applied Physics, California Institute of Technology, Pasadena, California, United States of America, Division of Biology, California Institute of Technology, Pasadena, California, United States of America
⨯

Tuning Promoter Strength through RNA Polymerase Binding Site Design in Escherichia coli

Robert C. Brewster,
Daniel L. Jones,
Rob Phillips

Published: December 13, 2012
https://doi.org/10.1371/journal.pcbi.1002811

About the Authors

Robert C. Brewster

Contributed equally to this work with: Robert C. Brewster, Daniel L. Jones

Affiliation Department of Applied Physics, California Institute of Technology, Pasadena, California, United States of America

Daniel L. Jones

Contributed equally to this work with: Robert C. Brewster, Daniel L. Jones

Affiliation Department of Applied Physics, California Institute of Technology, Pasadena, California, United States of America

Rob Phillips

* E-mail: phillips@pboc.caltech.edu

Affiliations Department of Applied Physics, California Institute of Technology, Pasadena, California, United States of America, Division of Biology, California Institute of Technology, Pasadena, California, United States of America

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: RCB DLJ RP. Performed the experiments: RCB DLJ. Analyzed the data: RCB DLJ. Contributed reagents/materials/analysis tools: RCB DLJ. Wrote the paper: RCB DLJ RP.

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Gene expression
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Transcription factors
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Thermodynamics
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Fluorescence imaging
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Messenger RNA
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DNA transcription
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Protein expression
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RNA polymerase
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