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Research Article

Modeling Bi-modality Improves Characterization of Cell Cycle on Gene Expression in Single Cells

  • Andrew McDavid equal contributor,

    equal contributor Contributed equally to this work with: Andrew McDavid, Lucas Dennis

    Affiliations: Department of Statistics, University of Washington, Seattle, Washington, United States of America, Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America

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  • Lucas Dennis equal contributor,

    equal contributor Contributed equally to this work with: Andrew McDavid, Lucas Dennis

    Affiliation: NanoString Technologies, Seattle, Washington, United States of America

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  • Patrick Danaher,

    Affiliation: NanoString Technologies, Seattle, Washington, United States of America

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  • Greg Finak,

    Affiliation: Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America

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  • Michael Krouse,

    Affiliation: NanoString Technologies, Seattle, Washington, United States of America

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  • Alice Wang,

    Affiliation: BD Biosciences, San Jose, California, United States of America

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  • Philippa Webster,

    Affiliation: NanoString Technologies, Seattle, Washington, United States of America

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  • Joseph Beechem,

    Affiliation: NanoString Technologies, Seattle, Washington, United States of America

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  • Raphael Gottardo mail

    raph@rglab.org

    Affiliations: Department of Statistics, University of Washington, Seattle, Washington, United States of America, Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America

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  • Published: July 17, 2014
  • DOI: 10.1371/journal.pcbi.1003696

About the Authors

Andrew McDavid, Raphael Gottardo
Department of Statistics, University of Washington, Seattle, Washington, United States of America
Andrew McDavid, Greg Finak, Raphael Gottardo
Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America
Lucas Dennis, Patrick Danaher, Michael Krouse, Philippa Webster, Joseph Beechem
NanoString Technologies, Seattle, Washington, United States of America
Alice Wang
BD Biosciences, San Jose, California, United States of America

Corresponding Author

Email: raph@rglab.org

Competing Interests

I have read the journal's policy and have the following conflicts: NanoStrings Technologies and BD Biosciences products were used to generate data for this manuscript. Lucas Dennis, Patrick Danaher, Michael Krouse, Philippa Webster and Joseph Beechem are employees of NanoString Technologies. Alice Wang is an employee of BD Biosciences.

Author Contributions

Conceived and designed the experiments: RG LD JB PW. Performed the experiments: LD AM AW MK. Analyzed the data: AM GF PD. Contributed reagents/materials/analysis tools: AW. Wrote the paper: AM RG PD LD GF.