NCBI identifier	Name	Sensitivity (%)	Stem length	Loop size	-Delta G [kcal/mole]	-Delta G / stemloop length [kcal/mole/nucleotide]	Number of Ts in the T-stretch

Bacillales; Bacillaceae; Bacillus

NC_000964	Bacillus subtilis subsp. subtilis str. 168	97.18	9.1	5.2	14.9	0.644	9.3
NC_006322	Bacillus licheniformis ATCC 14580	95.14	9.9	5.3	15.8	0.637	9.1
NC_006270	Bacillus licheniformis ATCC 14580	94.95	9.8	5.3	15.8	0.640	9.1
NC_003997	Bacillus anthracis str. Ames	96.35	9.8	5.2	14.8	0.602	9.6
NC_007530	Bacillus anthracis str. 'Ames Ancestor'	96.35	9.7	5.2	14.8	0.603	9.6
NC_005945	Bacillus anthracis str. Sterne	97.33	9.7	5.2	14.8	0.603	9.6
NC_005957	Bacillus thuringiensis serovar konkunian str. 97-27	96.88	9.9	5.2	14.9	0.602	9.6
NC_003909	Bacillus cereus ATCC 10987	94.53	9.7	5.3	14.7	0.596	9.6
NC_004722	Bacillus cereus ATCC 14579	95.85	9.9	5.1	15.1	0.609	9.6
NC_006274	Bacillus cereus ZK	97.24	9.7	5.0	14.7	0.604	9.6
NC_002570	Bacillus halodurans	84.34	10.4	5.6	14.9	0.563	9.1
NC_006582	Bacillus clausii KSM-K16	87.15	10.3	5.5	15.7	0.608	8.8

Bacillales; Bacillaceae; Geobacillus

NC_006510	Geobacillus kaustophilus HTA426	86.39	11.1	5.7	16.9	0.609	8.5

Bacillales; Bacillaceae; Oceanobacillus

NC_004193	Oceanobacillus iheyensis HTE831	88.30	11.5	5.9	14.9	0.514	9.5

Bacillales; Listeriaceae; Listeria

NC_002973	Listeria monocytogenes str. 4b F2365	97.47	11.1	5.2	16.8	0.613	10.0
NC_003210	Listeria monocytogenes EG	97.16	11.2	5.3	17.2	0.625	10.0
NC_003212	Listeria innocua Clip11262	98.16	10.9	5.3	16.7	0.617	10.0

Bacillales; Staphylococcus

NC_002953	Staphylococcus aureus subsp. aureus MSSA476	90.40	13.6	6.2	17.0	0.503	9.7
NC_002758	Staphylococcus aureus subsp. aureus Mu50	93.45	13.3	6.3	16.7	0.501	9.7
NC_002952	Staphylococcus aureus subsp. aureus MRSA252	92.15	13.9	6.0	17.5	0.512	9.7
NC_003923	Staphylococcus aureus subsp. aureus MW2	91.73	13.5	6.2	16.8	0.502	9.7
NC_002745	Staphylococcus aureus subsp. aureus N315	93.75	13.3	6.3	16.9	0.505	9.7
NC_002951	Staphylococcus aureus subsp. aureus COL	91.36	13.9	6.0	17.2	0.503	9.7
NC_004461	Staphylococcus epidermidis ATCC 12228	92.43	12.7	6.3	16.5	0.513	9.6
NC_002976	Staphylococcus epidermidis RP62A	91.40	12.6	6.1	16.4	0.513	9.6

Clostridia; Clostridiales; Clostridiaceae; Clostridium

NC_003366	Clostridium perfringens str. 13	94.15	12.0	5.5	13.9	0.472	10.0
NC_004557	Clostridium tetani E88	93.91	10.6	5.0	13.5	0.504	9.9
NC_003030	Clostridium acetobutylicum ATCC 824	94.30	10.8	6.0	13.3	0.478	9.8

Clostridia; Thermoanaerobacteriales; hermoanaerobacteriaceae; Thermoanaerobacter

NC_003869	Thermoanaerobacter tengcongensis MB4	87.55	9.7	5.9	13.5	0.545	9.5

Lactobacillales; Lactobacillaceae; Lactobacillus

NC_004567	Lactobacillus plantarum WCFS1	90.65	9.1	5.9	13.5	0.545	9.5
NC_005362	Lactobacillus johnsonii NCC 533	97.18	11.1	5.5	15.6	0.569	9.8
NC_006814	Lactobacillus acidophilus NCFM	96.15	10.2	5.2	13.8	0.547	9.9

Lactobacillales; Streptococcaceae; Streptococcus

NC_004116	Streptococcus agalactiae 2603V/R	96.93	9.1	4.8	12.4	0.541	9.8
NC_004368	Streptococcus agalactiae NEM316	98.77	8.9	4.9	12.6	0.554	9.8
NC_004350	Streptococcus mutans UA159	95.00	11.1	6.3	13.3	0.459	9.6
NC_003098	Streptococcus pneumoniae R6	94.76	10.6	5.3	13.8	0.525	9.3
NC_003028	Streptococcus pneumoniae TIGR4	92.48	10.8	5.5	14.3	0.530	9.2
NC_002737	Streptococcus pyogenes M1 GAS	95.77	10.5	5.4	14.2	0.530	9.6
NC_004070	Streptococcus pyogenes MGAS315	95.63	10.4	5.5	13.9	0.527	9.6
NC_004606	Streptococcus pyogenes SSI-1	94.37	10.7	5.3	13.8	0.520	9.6
NC_003485	Streptococcus pyogenes strain MGAS8232	93.33	11.2	5.3	14.8	0.539	9.6
NC_006086	Streptococcus pyogenes MGAS10394	94.47	10.5	5.4	14.2	0.536	9.6
NC_006449	Streptococcus thermophilus CNRZ1066	94.76	9.7	5.2	12.4	0.520	9.6
NC_006448	Streptococcus thermophilus LMG 18311	94.35	9.6	5.2	12.3	0.519	9.5

Lactobacillales; Streptococcaceae; Lactococcus

NC_002662	Lactococcus lactis subsp. lactis Il1403	99.30	8.3	5.1	10.9	0.511	10.5

Lactobacillales; Enterococcaceae; Enterococcus

NC_004668	Enterococcus faecalis V583	96.93	11.9	5.5	18.2	0.610	9.9

Mollicutes; Acholeplasmatales; Acholeplasmataceae; Candidatus Phytoplasma; Candidatus Phytoplasma asteris

NC_005303	Onion yellows phytoplasma OY-M	97.37	9.1	5.0	9.7	0.454	10.5

Mollicutes; Entomoplasmatales; Entomoplasmataceae

NC_006055	Mesoplasma florum L1	100	7.4	5.3	8.0	0.407	10.9

Mollicutes; Mycoplasmataceae; Mycoplasma

NC_004829	Mycoplasma gallisepticum R	94.94	10.3	5.6	12.7	0.497	10.3
NC_000908	Mycoplasma genitalium G-37	90.20	9.3	5.8	7.1	0.307	10.1
NC_006360	Mycoplasma hyopneumoniae 232	99.13	9.4	6.5	6.8	0.276	10.7
NC_006908	Mycoplasma mobile 163K	98.13	7.7	5.0	7.3	0.377	10.7
NC_005364	Mycoplasma mycoides subsp. mycoides SC str. PG1	94.56	10.2	5.2	9.6	0.395	10.7
NC_004432	Mycoplasma penetrans HF-2	99.25	10.4	6.0	10.6	0.408	10.7
NC_000912	Mycoplasma pneumoniae M129	82.89	9.5	5.7	10.0	0.424	9.4
NC_002771	Mycoplasma pulmonis UAB CTIP	100	9.5	6.3	8.6	0.352	10.8

Mollicutes; Mycoplasmataceae, Ureaplasma

NC_002162	Ureaplasma parvum serovar 3 str. ATCC 700970	96.55	9.6	5.7	9.3	0.380	10.7