ࡱ > ~ q` Z bjbjqPqP . : : (f 2 | 2 c* ) ) ) ) ) ) ) $ O+ h - v ) Q 6 6 6 ) * R" R" R" 6 2 % R" 6 ) R" R" R" ] h! R" " 3* 0 c* R" -. ~! -. R" -. R" @ 0 " R" 8 ) ) 0" " c* 6 6 6 6 2 2 d
2 2 2 2 2 Table S2. Temporal information known from experimental observations in B. bronchiseptica (Bb) and B. pertussis (Bp).
Node SpeciesPeak/first activityDescription/ SignificanceReferencePICBb/Bp2-24 hoursPro-inflammatory cytokines respond to the pathogen invasion in the first 24 hours. ADDIN EN.CITE Byrne200432032Byrne, P.McGuirk, P.Todryk, S.Mills, K. H.2004Depletion of NK cells results in disseminating lethal infection with Bordetella pertussis associated with a reduction of antigen-specific Th1 and enhancement of Th2, but not Tr1 cellsEur J Immunol3492579-88Sep15307190AnimalsAntibodies, Bacterial/biosynthesisBordetella pertussis/immunologyG(M1) Ganglioside/immunologyImmunoglobulin G/biosynthesisInterferon Type II/biosynthesisInterleukin-12/biosynthesisInterleukins/biosynthesisKiller Cells, Natural/*immunologyLung/immunologyLymphocyte DepletionMiceMice, Inbred BALB CResearch Support, Non-U.S. Gov'tTh1 Cells/*immunologyTh2 Cells/*immunologyWhooping Cough/*immunologyhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15307190
file://C:%5CDocuments%20and%20Settings%5CJuilee%20Thakar%5CDesktop%5CBordetella%20modeling%20paper%205.16.06.docImmune Regulation Research Group, Department of Biochemistry, Trinity College, Dublin, Ireland.[1]Recruited PMNsBb12 hoursFirst peak of recruited PMNs is at 12 hours. This is a nonspecific innate immune response. ADDIN EN.CITE Mann20044041550179872112004NovToll-like receptor 4-dependent early elicited tumor necrosis factor alpha expression is critical for innate host defense against Bordetella bronchiseptica6650-8Immunology Research Laboratories, The Pathobiology Graduate Program, Department of Veterinary Science, The Pennsylvania State University, University Park 16802, USA.Mann, P. B.Elder, K. D.Kennett, M. J.Harvill, E. T.Infect ImmunAnimalsBordetella Infections/*immunology/microbiologyBordetella bronchiseptica/*immunology/pathogenicityGene Expression Profiling*Gene Expression RegulationImmunity, NaturalMacrophages/immunology/metabolismMembrane Glycoproteins/genetics/*metabolismMiceMice, Inbred C57BLOligonucleotide Array Sequence AnalysisPneumonia, Bacterial/immunology/microbiologyProteins/genetics/metabolismReceptors, Cell Surface/genetics/*metabolismResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.Tumor Necrosis Factor-alpha/genetics/immunology/*metabolismhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15501798[2]Necrosis of recruited PMNsBb3-4 daysIn B. bronchiseptica the TTSS induces necrosis of PMNs between the 3rd and 4th day. This indicates a delay in the action of TTSS after bacterial invasion. ADDIN EN.CITE Stockbauer20033403412580948522003FebBordetella type III secretion induces caspase 1-independent necrosis123-32Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine at the University of California, Los Angeles, 10833 Le Conte Ave, Los Angeles, CA 90095-1747, USA.Stockbauer, K. E.Foreman-Wykert, A. K.Miller, J. F.Cell MicrobiolAnimalsBacterial Toxins/antagonists & inhibitors/*metabolismBordetella bronchiseptica/metabolism/*pathogenicityCaspase 1/genetics/physiology*Cell DeathCell LineComparative StudyCyclosporine/pharmacologyGlycine/pharmacologyHela CellsHumansMacrophages/*microbiology/pathologyMiceMice, Inbred C57BLMice, KnockoutNecrosisResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12580948Yuk200061061107126823552000MarModulation of host immune responses, induction of apoptosis and inhibition of NF-kappaB activation by the Bordetella type III secretion system991-1004Department of Microbiology and Immunology and Molecular Biology Institute, UCLA School of Medicine, Los Angeles, CA 90095-1747, USA.Yuk, M. H.Harvill, E. T.Cotter, P. A.Miller, J. F.Mol MicrobiolAnimalsAntibodies, Bacterial/*biosynthesis*ApoptosisBacterial Proteins/geneticsBase SequenceBordetella bronchiseptica/immunology/isolation &purification/*pathogenicityDNA PrimersEpithelial Cells/microbiologyFemaleMiceMice, Inbred BALB CMice, Inbred C57BLMice, SCIDNF-kappa B/*antagonists & inhibitors/metabolismResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.Respiratory System/microbiologyVirulencehttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10712682[3,4]Start of phagocytosis Bb12 hoursNon-specific phagocytosis starts12 hours after infection. ADDIN EN.CITE Mann20044041550179872112004NovToll-like receptor 4-dependent early elicited tumor necrosis factor alpha expression is critical for innate host defense against Bordetella bronchiseptica6650-8Immunology Research Laboratories, The Pathobiology Graduate Program, Department of Veterinary Science, The Pennsylvania State University, University Park 16802, USA.Mann, P. B.Elder, K. D.Kennett, M. J.Harvill, E. T.Infect ImmunAnimalsBordetella Infections/*immunology/microbiologyBordetella bronchiseptica/*immunology/pathogenicityGene Expression Profiling*Gene Expression RegulationImmunity, NaturalMacrophages/immunology/metabolismMembrane Glycoproteins/genetics/*metabolismMiceMice, Inbred C57BLOligonucleotide Array Sequence AnalysisPneumonia, Bacterial/immunology/microbiologyProteins/genetics/metabolismReceptors, Cell Surface/genetics/*metabolismResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.Tumor Necrosis Factor-alpha/genetics/immunology/*metabolismhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15501798[2]Dendritic cellsBp4-7 days
15-21 daysIn B. pertussis two peaks of dendritic cells are observed, supporting the differentiation of distinct subsets of T helper cells from T0 cells. ADDIN EN.CITE Byrne200432032Byrne, P.McGuirk, P.Todryk, S.Mills, K. H.2004Depletion of NK cells results in disseminating lethal infection with Bordetella pertussis associated with a reduction of antigen-specific Th1 and enhancement of Th2, but not Tr1 cellsEur J Immunol3492579-88Sep15307190AnimalsAntibodies, Bacterial/biosynthesisBordetella pertussis/immunologyG(M1) Ganglioside/immunologyImmunoglobulin G/biosynthesisInterferon Type II/biosynthesisInterleukin-12/biosynthesisInterleukins/biosynthesisKiller Cells, Natural/*immunologyLung/immunologyLymphocyte DepletionMiceMice, Inbred BALB CResearch Support, Non-U.S. Gov'tTh1 Cells/*immunologyTh2 Cells/*immunologyWhooping Cough/*immunologyhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15307190
file://C:%5CDocuments%20and%20Settings%5CJuilee%20Thakar%5CDesktop%5CBordetella%20modeling%20paper%205.16.06.docImmune Regulation Research Group, Department of Biochemistry, Trinity College, Dublin, Ireland.[1]T0 cellsBb3-7 daysIn B. bronchiseptica T0 cells are first observed during the first week of infection. Our unpublished data, ADDIN EN.CITE Skinner2005550551617711117572005Oct 1Bordetella type III secretion modulates dendritic cell migration resulting in immunosuppression and bacterial persistence4647-52Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.Skinner, J. A.Pilione, M. R.Shen, H.Harvill, E. T.Yuk, M. H.J ImmunolAnimalsBacterial Proteins/physiologyBordetella Infections/*immunology/metabolism/*microbiologyBordetella bronchiseptica/growth & development/*immunology/pathogenicityCell Movement/*immunologyDendritic Cells/cytology/*immunologyDown-Regulation/immunology*ImmunosuppressionInterferon Type I/metabolismInterleukin-10/deficiency/genetics/metabolismMiceMice, Inbred C57BLMice, KnockoutResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.Respiratory Tract Infections/immunology/metabolism/microbiologyUp-Regulation/immunologyhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16177111[5]Th2 cellsBb8 daysTh2 cells are observed after the first peak of dendritic cells. ADDIN EN.CITE Skinner2005550551617711117572005Oct 1Bordetella type III secretion modulates dendritic cell migration resulting in immunosuppression and bacterial persistence4647-52Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.Skinner, J. A.Pilione, M. R.Shen, H.Harvill, E. T.Yuk, M. H.J ImmunolAnimalsBacterial Proteins/physiologyBordetella Infections/*immunology/metabolism/*microbiologyBordetella bronchiseptica/growth & development/*immunology/pathogenicityCell Movement/*immunologyDendritic Cells/cytology/*immunologyDown-Regulation/immunology*ImmunosuppressionInterferon Type I/metabolismInterleukin-10/deficiency/genetics/metabolismMiceMice, Inbred C57BLMice, KnockoutResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.Respiratory Tract Infections/immunology/metabolism/microbiologyUp-Regulation/immunologyhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16177111[5]IL10 (a Th2RC)Bb/Bp3-7 daysFirst peak of IL10 is observed during the initial interaction between dendritic cells and T0 cells. ADDIN EN.CITE Byrne200432032Byrne, P.McGuirk, P.Todryk, S.Mills, K. H.2004Depletion of NK cells results in disseminating lethal infection with Bordetella pertussis associated with a reduction of antigen-specific Th1 and enhancement of Th2, but not Tr1 cellsEur J Immunol3492579-88Sep15307190AnimalsAntibodies, Bacterial/biosynthesisBordetella pertussis/immunologyG(M1) Ganglioside/immunologyImmunoglobulin G/biosynthesisInterferon Type II/biosynthesisInterleukin-12/biosynthesisInterleukins/biosynthesisKiller Cells, Natural/*immunologyLung/immunologyLymphocyte DepletionMiceMice, Inbred BALB CResearch Support, Non-U.S. Gov'tTh1 Cells/*immunologyTh2 Cells/*immunologyWhooping Cough/*immunologyhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15307190
file://C:%5CDocuments%20and%20Settings%5CJuilee%20Thakar%5CDesktop%5CBordetella%20modeling%20paper%205.16.06.docImmune Regulation Research Group, Department of Biochemistry, Trinity College, Dublin, Ireland.[1]AntibodiesBb8-14 daysAntibodies are first observed on the 8th day, that is, after the activation of Th2 cells. ADDIN EN.CITE Kirimanjeswara200319019126547847142003AprRole of antibodies in immunity to Bordetella infections1719-24Pathobiology Graduate Program, Department of Veterinary Science, The Pennsylvania State University, University Park, PA 16802, USA.Kirimanjeswara, G. S.Mann, P. B.Harvill, E. T.Infect ImmunAnimalsAntibodies, Bacterial/*blood/*immunologyB-Lymphocytes/immunologyBordetella/*immunologyBordetella Infections/*immunology/microbiologyBordetella bronchiseptica/immunologyBordetella pertussis/immunologyDisease Models, AnimalHumansImmune Sera/immunologyImmunityMiceMice, Inbred BALB CResearch Support, U.S. Gov't, Non-P.H.S.Respiratory System/immunology/microbiologyhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12654784[6]AgAb ComplexBb9-21 daysAntigen-antibody complexes are observed between the 9th and 21st day. ADDIN EN.CITE Kirimanjeswara200319019126547847142003AprRole of antibodies in immunity to Bordetella infections1719-24Pathobiology Graduate Program, Department of Veterinary Science, The Pennsylvania State University, University Park, PA 16802, USA.Kirimanjeswara, G. S.Mann, P. B.Harvill, E. T.Infect ImmunAnimalsAntibodies, Bacterial/*blood/*immunologyB-Lymphocytes/immunologyBordetella/*immunologyBordetella Infections/*immunology/microbiologyBordetella bronchiseptica/immunologyBordetella pertussis/immunologyDisease Models, AnimalHumansImmune Sera/immunologyImmunityMiceMice, Inbred BALB CResearch Support, U.S. Gov't, Non-P.H.S.Respiratory System/immunology/microbiologyhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12654784[6]IFN - ( a T h 1 R C ) B b 4 9 - 7 0 d a y s I F N - i s p r o d u c e d l a t e r , a r o u n d d a y 4 9 , i n B . b r o n c h i s e p t i c a . A D D I N E N . C I T E <